The Biotin-PRMT5/MEP50 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the Biotin-PRMT5/MEP50 protein, SAM and substrate at 25℃ for 180 min, then adding MTase-Glo reagent and reading RFU with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | O14744-1、Q9BQA1-1 |
Amino Acid Sequence | PRMT5: A2-L637(end); N-terminal Flag and Avi tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | PRMT5: 75.6 kDa; MEP50: 37.8 kDa |
Purity | >95% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | Avi Tag, His Tag, Flag Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
