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Biotin-Kras[G12C] Protein, Human

Biotin-Kras[G12C] Protein, Human

Catalog Number: UA080462 Brand: UA BIOSCIENCE
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Product Details

Product Specification


Species Human
Synonyms GTPase KRas,KRAS2,RASK2
Accession P01116-2
Amino Acid Sequence

M1-K169,with N-terminal 8x His tag-TEV, C-terminal Avi
Expression System E.coli
Molecular Weight 23.6 kDa
Purity >90% by SDS-PAGE
Conjugation Unconjugated
Tag Avi Tag, His Tag
Physical Appearance Liquid
Storage Buffer

50 mM Tris, 150 mM NaCl, 10% glycerol, pH 7.5
Stability & Storage

Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles.

Picture

Bioactivity

The Biotin-KRAS[G12C] activity was quantified using a HTRF-based assay. The reaction was performed by incubating the Biotin-KRAS[G12C] protein and substrate, the donor and receptor at 25℃ for 60 min, then measuring the 665nm/615nm fluorescence signal ratio on BMG.

SDS-PAGE