WB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
VDAC2 Rabbit Polyclonal Antibody
VDAC2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VDAC2 |
| Synonyms | Non-selective voltage-gated ion channel VDAC2; VDAC-2; hVDAC2; Outer mitochondrial membrane protein porin 2 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P45880 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | COS-7, HEK-293, HepG2, Jurkat, RAW264.7, mouse brain, PC-12, rat heart |
| Predicted Reactivity | Hm, Rb, Pg, Bv, Tk |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
VDAC2 (Voltage-Dependent Anion Channel 2) is an evolutionarily conserved, ~32 kDa integral β-barrel porin embedded in the outer mitochondrial membrane where it orchestrates the flux of ATP, ADP, metabolites, and ions between the cytosol and mitochondria, serves as a crucial docking platform for pro- and anti-apoptotic Bcl-2 family proteins to modulate cytochrome-c release and intrinsic apoptosis, interacts with hexokinases to couple glycolysis to oxidative phosphorylation in cancer metabolism, and responds to transmembrane potential and pharmacological effectors such as erastin in ferroptosis; its expression is nearly ubiquitous yet highest in heart, skeletal muscle, and brain, and dysregulation contributes to neurodegeneration, cardiomyopathy, and chemoresistance, making VDAC2 a convergent therapeutic target for metabolic reprogramming and cell-death modulation.
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Western Blot
WB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
WB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
WB result of VDAC2 Rabbit pAb
Primary antibody: VDAC2 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 31 kDa
Observed MW: 32 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-VDAC2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
