Thrombomodulin Recombinant Rabbit mAb (SDT-153-89)

Catalog Number: S0B0056 Application: WB,IHC-P,ICC,FCM,IP Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25 μl 100 μl 1 ml

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Product Details

Product Specification

Host	Rabbit
Antigen	Thrombomodulin
Synonyms	TM, Fetomodulin, CD141
Immunogen	Recombinant Protein
Location	Membrane
Accession	P07204
Clone Number	SDT-153-89
Antibody Type	Rabbit mAb
Application	WB, IHC-P, ICC, ICFCM, IP
Reactivity	Hu
Purification	Protein A
Concentration	0.25 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:5000	null
IHC-P	1:1000	null
IP	1:25	null
ICFCM	1:250	null
ICC	1:250	null

Background

Thrombomodulin (TM) is an integral component of a multimolecular system, localized primarily to the vascular endothelium, that integrates crucial biological processes and biochemical pathways, including those related to coagulation, innate immunity, inflammation, and cell proliferation. These are designed to protect the host from injury and promote healing [PMID: 29866818].

Picture

Western Blot

WB result of Thrombomodulin Rabbit mAb
Primary antibody: Thrombomodulin Rabbit mAb at 1/5000 dilution
Lane 1: A431 whole cell lysate 5 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 60 kDa
Observed MW: 90 kDa

FC

Flow cytometric analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labelling Thrombomodulin antibody at 1/250 dilution (0.1 μg)/ (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.

IP

Thrombomodulin Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating Thrombomodulin in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using Thrombomodulin Rabbit mAb at 1/5000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A431 whole cell ly-sate 10 µg (Input)
Lane 2: Thrombomodulin Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 60 kDa
Observed MW: 90 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human cervcial squamous cell carcinoma. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-Thrombomodulin antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in A431 cells. Anti-Thrombomodulin antibody was used at 1/250 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanoland permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).