WB result of TAK1 Recombinant Rabbit mAb
Primary antibody: TAK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 78 kDa
TAK1 Recombinant Rabbit mAb (S-2482-39)
TAK1 Recombinant Rabbit mAb (S-2482-39)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TAK1 |
| Synonyms | Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 (TGF-beta-activated kinase 1); MAP3K7 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | O43318 |
| Clone Number | S-2482-39 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HeLa, A431, A549, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt, Mk |
Background
TAK1 (MAP3K7) is a ubiquitously expressed serine/threonine kinase that acts as a central signaling hub by integrating cues from TGF-β, inflammatory cytokines (TNF-α, IL-1), pattern-recognition receptors (TLR, NOD-like receptors) and cellular stress to catalyze the activation of IKK and MAP2Ks (MKK3/6, MKK4/7), thereby driving the NF-κB, p38, JNK and ERK pathways that collectively regulate inflammation, innate and adaptive immunity, cell survival, differentiation and metabolic adaptation; its activity is tightly controlled through stimulus-inducible assembly with regulatory binding partners TAB1, TAB2 and TAB3 and by polyubiquitin scaffolds, and genetic or pharmacologic dysregulation of TAK1 is linked to inflammatory diseases, autoimmunity and cancer.
Picture
Picture
Western Blot
WB result of TAK1 Recombinant Rabbit mAb
Primary antibody: TAK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 78 kDa
WB result of TAK1 Recombinant Rabbit mAb
Primary antibody: TAK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 78 kDa
WB result of TAK1 Recombinant Rabbit mAb
Primary antibody: TAK1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 67 kDa
Observed MW: 78 kDa
