STING Recombinant Rabbit mAb (SDT-R150)

Catalog Number: S0B0084 Application: WB,IHC-P,IP Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100 μl

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Product Details

Product Specification

Host	Rabbit
Antigen	STING
Synonyms	Hsting, ERIS, hMITA, Transmembrane protein 173
Immunogen	N/A
Location	Cytoplasm, Cell membrane, Endoplasmic reticulum membrane, Mitochondrion outer membrane
Accession	Q86WV6
Clone Number	SDT-R150
Antibody Type	Recombinant mAb
Application	WB, IHC-P, IP
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	null
IHC-P	1:500	null
IP	1:50	null

Background

Mediator of IRF3 activation (MITA, also known as stimulator of interferon genes (STING) and endoplasmic reticulum interferon stimulator (ERIS)) is an ER-associated protein that senses cellular and bacterium-derived cyclic dinucleotide (CDN), leading to induction of type-I interferons (IFNs) and innate immune responses against viruses and bacteria [PMID: 36193584].

Picture

Western Blot

WB result of STING Rabbit mAb Primary antibody: STING Rabbit mAb at 1/1000 dilution Lane 1: THP-1 whole cell lysate 20 µg Lane 2: HaCaT whole cell lysate 20 µg Lane 3: HT-29 whole cell lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 42kDa Observed MW: 30, 37kDa

IP

STING Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating STING in 0.4 mg HL-60 whole cell lysate.
Western blot was performed on the immunoprecipitate using STING Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HL-60 whole cell lysate 20 µg (Input)
Lane 2: STING Rabbit mAb IP in HL-60 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HL-60 whole cell lysate
Predicted MW: 42 kDa
Observed MW: 37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-STING antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.