WB result of Stat5b Recombinant Rabbit mAb
Primary antibody: Stat5b Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Lane 6: Daudi whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 80 kDa
Stat5b Recombinant Rabbit mAb (S-2951-34)
Stat5b Recombinant Rabbit mAb (S-2951-34)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Stat5b |
| Synonyms | Signal transducer and activator of transcription 5B |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P42232 |
| Clone Number | S-2951-34 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | K562, HeLa, Jurkat, Raji, Daudi, NIH/3T3, RAW264.7, mouse heart, PC-12, rat heart |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
Background
Stat5b (signal transducer and activator of transcription 5b) is a 787-amino-acid transcription factor that, upon phosphorylation by JAK kinases in response to cytokines such as IL-2, GH, prolactin or IFN-α, dimerizes and translocates to the nucleus where it binds the consensus GAS motif TTCNNNGAA to regulate genes controlling cell survival, proliferation, differentiation and metabolic reprogramming; it is indispensable for normal immune homeostasis, mammary gland development and liver GH signaling, and its persistent activation is a driver of multiple hematologic and solid cancers, making Stat5b a validated therapeutic target.
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Western Blot
WB result of Stat5b Recombinant Rabbit mAb
Primary antibody: Stat5b Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 80 kDa
WB result of Stat5b Recombinant Rabbit mAb
Primary antibody: Stat5b Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 80 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-stat5b antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in RAW264.7 cells. Anti-Stat5b antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
