WB result of SQLE Recombinant Rabbit mAb
Primary antibody: SQLE Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Karpas-299 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Negative control: Karpas-299 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 55-64 kDa
SQLE Recombinant Rabbit mAb (S-2358-207)
SQLE Recombinant Rabbit mAb (S-2358-207)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SQLE |
| Synonyms | Squalene monooxygenase; Squalene epoxidase (SE); ERG1 |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | Q14534 |
| Clone Number | S-2358-207 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Mk |
| Positive Sample | HEK-293, Raji, PC-3, A549, HepG2, F9, mouse testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
SQLE (squalene epoxidase or squalene monooxygenase) is a 64 kDa, 574-residue FAD-dependent endoplasmic-reticulum enzyme that catalyzes the stereospecific epoxidation of squalene to 2,3-oxidosqualene, the first committed oxygenation and second rate-limiting step of cholesterol biosynthesis; it comprises an N-terminal cholesterol-sensing regulatory domain that undergoes MARCH6-mediated ubiquitination and proteasomal degradation when membrane cholesterol is high, and a C-terminal catalytic domain that uses NADPH-cytochrome P450 reductase to transfer electrons for the reaction, while its own substrate squalene can bind the regulatory domain to suppress degradation, thus functioning as both feed-forward signal and metabolite, and because fungal SQLE is sufficiently divergent, selective inhibitors such as terbinafine block fungal ergosterol synthesis without affecting the human enzyme, making SQLE a validated antimicrobial target.
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Western Blot
WB result of SQLE Recombinant Rabbit mAb
Primary antibody: SQLE Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: F9 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 64 kDa
Observed MW: 64 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-SQLE antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti- SQLE antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
