SPARC Recombinant Rabbit mAb (SDT-R136)

Catalog Number: S0B0066 Application: WB,IHC-P,IP Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100 μl

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Product Details

Product Specification

Host	Rabbit
Antigen	SPARC
Synonyms	BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine
Immunogen	N/A
Location	Secreted, Extracellular matrix, Basement membrane
Accession	P09486
Clone Number	SDT-R136
Application	WB, IHC-P, IP
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	null
IHC-P	1:2000	null
IP	1:50	null

Background

SPARC, also referred to as osteonectin or basement membrane protein 40 (BM-40), is a ~32 kDa calcium-binding matricellular protein [PMID: 22249026, PMID: 25064829]. At one time SPARC was thought to be expressed specifically in mineralized tissues (thus the name osteonectin) but was subsequently demonstrated to have a much wider expression pattern in both mineralized and non-mineralized tissues [PMID: 7034958]. Typically, expression of SPARC is closely aligned with that of fibrillar collagens such as collagen type I. The protein component of the pre-mineralized bone matrix (osteoid) is composed mainly of collagen type I (94%) and a number of noncollagenous proteins.

Picture

Western Blot

WB result of SPARC Rabbit mAb Primary antibody: SPARC Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: SH-SY5Y whole cell lysate 20 µg Lane 3:A-375 whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 35 kDa Observed MW: 37 kDa

IP

SPARC Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SPARC in 0.4 mg A375 whole cell lysate.
Western blot was performed on the immunoprecipitate using SPARC Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: A375 whole cell lysate 20 µg (Input)
Lane 2: SPARC Rabbit mAb IP in A375 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A375 whole cell lysate
Predicted MW: 35 kDa
Observed MW: 37 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human kidney. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human testis. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-SPARC antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.