WB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HCT 116 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
SMAD2 Recombinant Rabbit mAb (S-1521-177)
SMAD2 Recombinant Rabbit mAb (S-1521-177)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SMAD2 |
| Synonyms | Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2 (hMAD-2); SMAD family member 2 (SMAD 2; Smad2; hSMAD2); MADH2; MADR2 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q15796 |
| Clone Number | S-1521-177 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HCT 116, HEK-293, mouse brain, C6, rat brain |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SMAD2 is a key protein in the TGF-β signaling pathway, which plays a crucial role in regulating various cellular processes such as cell growth, differentiation, and immune responses. When TGF-β binds to its receptors, it activates the receptor complex, leading to the phosphorylation of SMAD2. Phosphorylated SMAD2 then forms a complex with SMAD4 and other cofactors, acting as a transcription factor to regulate gene expression. This pathway is essential for maintaining cellular homeostasis, but dysregulation of SMAD2 and TGF-β signaling has been implicated in diseases such as fibrosis, cancer, and atherosclerosis.
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Picture
Western Blot
WB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
WB result of SMAD2 Recombinant Rabbit mAb
Primary antibody: SMAD2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 58 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SMAD2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti- SMAD2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
