WB result of Sirt3 Recombinant Rabbit mAb
Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 26 kDa
Sirt3 Recombinant Rabbit mAb (S-2004-6)
Sirt3 Recombinant Rabbit mAb (S-2004-6)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Sirt3 |
| Synonyms | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; hSIRT3; NAD-dependent protein delactylase sirtuin-3; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; SIR2L3; SIRT3 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q9NTG7 |
| Clone Number | S-2004-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | EK-293, PANC-1, mouse liver, rat liver, HaCAT |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| ICC | 1:100 | Hu |
Background
Sirt3, a member of the sirtuin family of proteins, is an important enzyme that is primarily located in the mitochondria. It functions as a deacetylase, removing acetyl groups from various proteins, which helps regulate their activity and stability. Sirt3 plays a crucial role in cellular metabolism, stress resistance, and aging processes. By modulating the activity of key metabolic enzymes, it helps maintain mitochondrial function and energy homeostasis. Additionally, Sirt3 is involved in protecting cells against oxidative stress by influencing the expression and activity of antioxidant enzymes. Its dysregulation has been implicated in various diseases, including metabolic disorders and cancer, making it a potential target for therapeutic interventions.
Picture
Picture
Western Blot
WB result of Sirt3 Recombinant Rabbit mAb
Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse spleen lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Negative control: mouse spleen lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 26 kDa
WB result of Sirt3 Recombinant Rabbit mAb
Primary antibody: Sirt3 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Lane 2: rat liver lysate 20 µg
Negative control: rat spleen lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 43 kDa
Observed MW: 26 kDa
Immunocytochemistry
ICC shows positive staining in HaCAT cells. Anti-Sirt3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
