WB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 120 kDa
SIRT1 Recombinant Rabbit mAb (S-1886-15)
SIRT1 Recombinant Rabbit mAb (S-1886-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SIRT1 |
| Synonyms | NAD-dependent protein deacetylase sirtuin-1; hSIRT1; NAD-dependent protein deacylase sirtuin-1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1 (hSIR2); SIR2L1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q96EB6 |
| Clone Number | S-1886-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HEK-293, HeLa, NIH/3T3, mouse testis, C6, rat testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Ms |
Background
SIRT1 protein, also known as Silent Information Regulator 1, is a NAD+-dependent deacetylase that plays a crucial role in various cellular processes. It is involved in the regulation of gene expression by histone deacetylation and modulates the acetylation levels of numerous substrates, including p53, PGC-1α, NF-κB, FOXO, NCoR, and p300. SIRT1 is essential for cell cycle control, energy metabolism, response to oxidative stress, cell apoptosis, and aging. Calorie restriction (CR) has been shown to increase SIRT1 expression, which is linked to extended lifespan in mammals. SIRT1 also has anti-inflammatory and anti-aging properties, and its dysregulation is associated with diseases such as mitochondrial complex I deficiency and aging. In the brain, SIRT1 upregulates tight junction proteins, maintaining the integrity of the blood-brain barrier. In the immune system, SIRT1 regulates the activation and differentiation of dendritic cells (DCs) and controls the phenotype and functions of DCs. SIRT1 is a promising target for drug design, with potential applications in treating various diseases, including cancer, neurodegenerative diseases, and inflammatory conditions.
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Western Blot
WB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 110 kDa
This blot was developed with high sensitivity substrate
WB result of SIRT1 Recombinant Rabbit mAb
Primary antibody: SIRT1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 110 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SIRT1 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NIH/3T3 cells. Anti- SIRT1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
