WB result of Siglec7 Recombinant Rabbit mAb
Primary antibody: Siglec7 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: PANC-1 whole cell lysate 20 µg
Lane 3: U937 whole cell lysate 20 µg
Lane 4: RPMI-8226 whole cell lysate 20 µg
Negative control: HeLa whole cell lysate; PANC-1 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 51 kDa
Observed MW: 60-100 kDa
Siglec7 Recombinant Rabbit mAb (S-2116-168)
Siglec7 Recombinant Rabbit mAb (S-2116-168)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD328 |
| Synonyms | Sialic acid-binding Ig-like lectin 7; Siglec-7; Adhesion inhibitory receptor molecule 1 (AIRM-1); CDw328; D-siglec; QA79 membrane protein; p75; CD328; AIRM1; SIGLEC7 |
| Immunogen | Recombinant Protein |
| Location | Membrane |
| Accession | Q9Y286 |
| Clone Number | S-2116-168 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | U937, RPMI-8226 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
CD328, also known as Siglec-7, is a 467-amino-acid inhibitory receptor of the CD33-related Siglec family that displays a single N-terminal V-set immunoglobulin domain for binding α2,3- and α2,6-linked sialic acids, followed by two C2-set Ig domains, a transmembrane segment and a cytoplasmic tail containing two ITIMs; expressed predominantly on NK cells, CD8⁺ T cells and monocytes, it recruits SHP-1/2 phosphatases upon engagement of sialylated glycans, thereby dampening cytotoxicity, cytokine release and cellular activation to help maintain immune tolerance.
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Picture
Western Blot
