WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 70 kDa
Scavenging Receptor SR-BI Recombinant Rabbit mAb (S-1736-15)
Scavenging Receptor SR-BI Recombinant Rabbit mAb (S-1736-15)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Scavenging Receptor SR-BI |
| Synonyms | Scavenger receptor class B member 1; SRB1; CD36 and LIMPII analogous 1 (CLA-1); CD36 antigen-like 1; Collagen type I receptor; thrombospondin receptor-like 1; SR-BI; CD36; CD36L1; CLA1; SCARB1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | Q8WTV0 |
| Clone Number | S-1736-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, THP-1, NIH/3T3, rat liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Scavenger receptor class B type I (SR-BI) is a versatile and physiologically significant HDL receptor that plays a crucial role in cholesterol homeostasis and lipoprotein metabolism. It is a cell surface glycoprotein highly expressed in the liver, steroidogenic tissues, endothelium, and other tissues. SR-BI mediates the selective uptake of cholesterol esters from HDL particles into cells through hydrophobic channels without internalizing the entire lipoprotein, facilitating reverse cholesterol transport. This process helps remove excess cholesterol from peripheral tissues and promotes its excretion via bile acids, thus exerting anti-atherosclerotic effects. Additionally, SR-BI is involved in various biological functions, including regulation of inflammation, endothelial cell function, and lipid metabolism in macrophages. Its dysfunction or genetic mutations are associated with increased cardiovascular risk and altered HDL metabolism.
Picture
Picture
Western Blot
WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 70 kDa
WB result of Scavenging Receptor SR-BI Recombinant Rabbit mAb
Primary antibody: Scavenging Receptor SR-BI Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 61 kDa
Observed MW: 90 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat liver. Anti-Scavenging Receptor SR-BI antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-Scavenging Receptor SR-BI antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
