WB result of SA2 (isoform-2) Recombinant Rabbit mAb
Primary antibody: SA2 (isoform-2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 146 kDa
Observed MW: 146 kDa
SA2 (isoform-2) Recombinant Rabbit mAb (S-1461-231)
SA2 (isoform-2) Recombinant Rabbit mAb (S-1461-231)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SA2 (isoform-2) |
| Synonyms | Cohesin subunit SA-2; SCC3 homolog 2; Stromal antigen 2; SA2; STAG2 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | Q8N3U4-2 |
| Clone Number | S-1461-231 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, K562, 293T, NIH/3T3, mouse spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| ICC | 1:50 | Hu, Ms |
Background
SA2 (isoform-2) protein is a variant of the spliceosome-associated protein family, playing a crucial role in pre-mRNA splicing. It is involved in the assembly and function of the spliceosome, a complex molecular machine that removes introns from pre-mRNA transcripts. This protein isoform is essential for accurate and efficient splicing, thereby ensuring proper gene expression. Mutations or dysregulation of SA2 can lead to various cellular defects and may be implicated in certain diseases.
Picture
Picture
Western Blot
WB result of SA2 (isoform-2) Recombinant Rabbit mAb
Primary antibody: SA2 (isoform-2) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 146 kDa
Observed MW: 146 kDa
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti-SA2 (isoform-2) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in NIH/3T3 cells. Anti-SA2 (isoform-2) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
