WB result of S100A13 Rabbit mAb
Primary antibody: S100A13 Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HUVEC whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 11 kDa
Observed MW: 13 kDa
S100A13 Recombinant Rabbit mAb (SDT-R040)
S100A13 Recombinant Rabbit mAb (SDT-R040)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | S100A13 |
| Synonyms | S100 calcium-binding protein A13 |
| Immunogen | N/A |
| Location | Cytoplasm, Secreted |
| Accession | Q99584 |
| Clone Number | SDT-R040 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.0625 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | null |
| WB | 1:1000 | null |
Background
S100A13 is a 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-Hand CA ion binding protein. S100A13 is widely expressed in various types of tissue, and high protein expression is detected in thyroid filter cells, testicular Leydig cells, and brain -specific cells. S100A13 can regulate the secretion of FGF1 and IL1α, and has potential to become a symbol for the production of tumors and endometrial vascular. CK19 and S100A13 in cPTC are significantly increased, indicating that S100A13 may be regarded as a new candidate PTC biomarker.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human brain.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human melanoma.Anti-S100A13 antibody was used at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
