WB result of Runx2 Recombinant Rabbit mAb
Primary antibody: Runx2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: LNCaP whole cell lysate 20 µg
Lane 3: Saos-2 whole cell lysate 20 µg
Lane 4: PC-3 whole cell lysate 20 µg
Lane 5: MDA-MB-231 whole cell lysate 20 µg
Lane 6: HeLa whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Low expression control: LNCaP whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50-70 kDa
Runx2 Recombinant Rabbit mAb (S-1767-173)
Runx2 Recombinant Rabbit mAb (S-1767-173)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Runx2 |
| Synonyms | Runt-related transcription factor 2; Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1 (CBF-alpha-1); Oncogene AML-3; Osteoblast-specific transcription factor 2 (OSF-2); Polyomavirus enhancer-binding protein 2 alpha A subunit (PEA2-alpha A; PEBP2-alpha A); AML3; CBFA1; OSF2; PEBP2A |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q13950 |
| Clone Number | S-1767-173 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Saos-2, PC-3, MDA-MB-231, C2C12 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Runx2, also known as Runt-related transcription factor 2, is a crucial transcription factor for bone development and plays a key role in the regulation of osteoblast differentiation and skeletal morphogenesis. It is expressed in prehypertrophic and hypertrophic chondrocytes, and its mutation can lead to skeletal malformation syndromes such as cleidocranial dysplasia. Runx2 induces chondrocyte maturation and proliferation through the regulation of various signaling pathways, including Indian hedgehog (Ihh) and vascular endothelial growth factor A (Vegfa). In addition to its role in normal bone development, Runx2 is also involved in pathological conditions such as breast cancer metastasis and lung fibrosis.
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Western Blot
WB result of Runx2 Recombinant Rabbit mAb
Primary antibody: Runx2 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 57 kDa
Observed MW: 50-70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic hyperplasia. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human osteosarcoma. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-Runx2 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in PC-3 cells (top panel) and negative staining in LNCaP cells (below panel). Anti-Runx2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
