WB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDa
ROCK2 Rabbit Polyclonal Antibody
ROCK2 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ROCK2 |
| Synonyms | Rho-associated protein kinase 2; Rho kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II (ROCK-II); p164 ROCK-2; KIAA0619 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane, Nucleus |
| Accession | O75116 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HepG2, HEK-293, MCF7, NIH/3T3, C2C12, mouse brain, PC-12, rat brain |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
ROCK2 (Rho-associated kinase 2) is a serine/threonine protein kinase that belongs to the Rho GTPase effector family and plays a crucial role in the regulation of the cytoskeleton dynamics, cell migration, proliferation, and survival. ROCK2 has been identified as a key driver in the development of various cancers. In acute myeloid leukemia (AML), increased expression of ROCK2 leads to lower rates of complete remission and poorer overall survival. Inhibition of ROCK2 can enhance the sensitivity of AML cell lines and primary AML samples to drugs and reduce the activity of downstream signaling pathways. ROCK2 is a key modulator in immune regulation and fibrosis and has become a therapeutic target for chronic graft-versus-host disease (cGVHD). ROCK2 plays a primary regulatory role in axonal degeneration, neuronal death, and axonal regeneration in the central nervous system (CNS). Downregulation of ROCK2 can inhibit apoptosis, reduce axonal degeneration, and promote neurite outgrowth.
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Western Blot
WB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: C2C12 whole cell lysate 20 µg
Lane 3: mouse brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDa
WB result of ROCK2 Rabbit pAb
Primary antibody: ROCK2 Rabbit pAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 161 kDa
Observed MW: 160 kDa
IP
ROCK2 Rabbit pAb at 1/50 dilution (1 µg) immunoprecipitating ROCK2 in 0.4 mg C2C12 whole cell lysate.
Western blot was performed on the immunoprecipitate using ROCK2 Rabbit pAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: C2C12 whole cell lysate 20 µg (Input)
Lane 2: ROCK2 Rabbit pAb IP in C2C12 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in C2C12 whole cell lysate
Predicted MW: 161 kDa
Observed MW: 160 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat colon. Anti-ROCK2 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-ROCK2 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
