WB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: HT-29 whole cell lysate 20 µg
Lane 2: HL-60 whole cell lysate 20 µg
Lane 3: Raji whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrate
RIPK1 Rabbit Polyclonal Antibody
RIPK1 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RIPK1 |
| Synonyms | Receptor-interacting serine/threonine-protein kinase 1; Cell death protein RIP; Receptor-interacting protein 1 (RIP-1); RIP; RIP1; RIPK1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cell membrane |
| Accession | Q13546 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HT-29, HL-60, Raji, HeLa, NIH/3T3, mouse kidney, mouse heart, C6, rat kidney |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
Background
RIPK1 (Receptor-Interacting Serine/Threonine-Protein Kinase 1) is a critical decision-maker of cell fate, a multifunctional protein that plays a central role at the crossroads of cell survival, inflammation, and death signaling pathways. Initially identified as a key component of the tumor necrosis factor receptor 1 (TNFR1) signaling complex, it performs dual roles through its kinase activity and scaffolding function: on one hand, it promotes the transcription of pro-survival genes and inflammatory responses by recruiting and activating downstream signaling molecules (such as the NF-κB and MAPK pathways); on the other hand, under specific conditions, RIPK1 can become a powerful driver of cell death, capable of activating caspase-8-mediated apoptosis or participating in the formation of a phosphorylated MLKL-mediated necroptosis complex. Consequently, the activity of RIPK1 is tightly regulated by various post-translational modifications, including ubiquitination, phosphorylation, and caspase-mediated cleavage. Dysregulation of its function is closely associated with numerous pathological processes such as sepsis, neurodegenerative diseases (like Alzheimer's disease and amyotrophic lateral sclerosis), autoimmune disorders, and ischemic injury.
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Western Blot
WB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse kidney lysate 20 µg
Lane 3: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrate
WB result of RIPK1 Rabbit pAb
Primary antibody: RIPK1 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat kidney lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 76 kDa
Observed MW: 47, 70 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-RIPK1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
