WB result of RFP Recombinant Rabbit mAb
Primary antibody: RFP Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with empty vector whole cell Lysate 20 µg
Lane 3: 293T transfected with Histone H3-RFP-Avi Tag whole cell Lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 48 kDa
RFP Recombinant Rabbit mAb (S-1006-109)
RFP Recombinant Rabbit mAb (S-1006-109)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RFP |
| Synonyms | Red fluorescent protein drFP583, DsRed |
| Immunogen | Recombinant Protein |
| Accession | Q9U6Y8 |
| Clone Number | S-1006-109 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:10000 | Species Independent |
| ICC | 1:500 | Species Independent |
Background
Red fluorescent protein, also known as RFP, is a type of protein that emits red fluorescence under ultraviolet light. It is an essential optical imaging tool in the field of life science. RFP can be used for live cell imaging, marking protein expression, and various protein-related experiments. It is one of the most commonly used fluorescent proteins, along with green fluorescent protein (GFP) and orange fluorescent protein (OFP). RFP has several advantages compared to GFP. Firstly, RFP can be used in combination with GFP to address scientific issues that GFP alone cannot solve. Secondly, RFP has longer excitation and emission wavelengths, which results in a lower background during intracellular imaging.
Picture
Picture
Western Blot
WB result of RFP Recombinant Rabbit mAb
Primary antibody: RFP Rabbit mAb at 1/10000 dilution
Lane 1: RFP protein 20 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 28 kDa
Immunocytochemistry
ICC shows negative staining in vector-transfected 293T cells. Anti-RFP antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
ICC shows positive staining in Histone H3-RFP-Avi Tag transfected 293T cells. Anti-RFP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
