WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Low expression control: U937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-30 kDa
RBPMS Recombinant Rabbit mAb (S-2387-235)
RBPMS Recombinant Rabbit mAb (S-2387-235)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RBPMS |
| Synonyms | RNA-binding protein with multiple splicing; RBP-MS; Heart and RRM expressed sequence (Hermes); HERMES |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q93062 |
| Clone Number | S-2387-235 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IF |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HepG2, A549, A431, HeLa, mouse heart, rat heart, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
| IF | 1:500 | Ms |
Background
RBPMS (RNA-binding protein with multiple splicing) is an RNA-recognition-motif protein that binds tandem CAC motifs in pre-mRNAs to orchestrate tissue-specific alternative splicing, mRNA stability and translational control; in the heart it regulates inclusion of sarcomeric exons (e.g., Ttn exon 47, Pdlim5, Nexn) essential for post-natal contractility, so its loss causes dilated cardiomyopathy and heart failure, while in retina and neurons it serves as a selective marker of retinal ganglion cells and modulates axonal integrity, and during development it governs cardiomyocyte cytokinesis, preventing premature binucleation and non-compaction cardiomyopathy, thereby exerting stage- and cell-type-specific control over RNA processing critical for vision, neural function and cardiac performance.
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Western Blot
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse skeletal muscle lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Low expression control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-26 kDa
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat heart lysate 20 µg
Low expression control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-30 kDa
WB result of RBPMS Recombinant Rabbit mAb
Primary antibody: RBPMS Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 22-26 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human stomach. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-RBPMS antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti-RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse retina. Anti-RBPMS antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
