WB result of RB1CC1 Recombinant Rabbit mAb
Primary antibody: RB1CC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A172 whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
RB1CC1 Recombinant Rabbit mAb (S-2367-251)
RB1CC1 Recombinant Rabbit mAb (S-2367-251)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RB1CC1 |
| Synonyms | RB1-inducible coiled-coil protein 1; FAK family kinase-interacting protein of 200 kDa (FIP200); KIAA0203; RBICC |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Lysosome |
| Accession | Q8TDY2 |
| Clone Number | S-2367-251 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A172, Jurkat, A20, mouse testis |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
RB1CC1 (also known as FIP200) is a multifunctional 183-kDa scaffolding protein that nucleates the ULK1-ATG13-RB1CC1-ATG101 complex to initiate autophagosome formation in response to nutrient or mTOR signals, acts in the nucleus as a GC-rich DNA-binding transcription factor that up-regulates RB1, p16 and p21 to enforce cell-cycle checkpoints, and simultaneously controls migration, differentiation and survival by inhibiting the focal-adhesion kinases PTK2/FAK1 and PTK2B/PYK2 and by modulating TSC1, p53 and TGF-β pathways, thereby operating as a central tumor-suppressor whose loss or mis-localation is linked to aggressive breast, prostate and pancreatic cancers as well as to fibrotic and neuro-developmental disorders.
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Picture
Western Blot
WB result of RB1CC1 Recombinant Rabbit mAb
Primary antibody: RB1CC1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 183 kDa
Observed MW: 180 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-RB1CC1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in Jurkat cells. Anti- RB1CC1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
