Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / HT29 (Human colorectal adenocarcinoma epithelial cell, Right) labelling CD324 antibody at 1/2000 dilution (0.1 μg) / (Red) compared with a Rat IgG1, κ Isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Negative control: MDA-MB-231
Rat Anti-Mouse/Human CD324 Antibody (S-R650)
Rat Anti-Mouse/Human CD324 Antibody (S-R650)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD324 |
| Synonyms | Cadherin-1; CAM 120/80; Epithelial cadherin (E-cadherinImported); Uvomorulin; CDHE; UVO; CDH1 |
| Location | Cytoplasm, Endosome, Cell membrane |
| Accession | P12830、P09803 |
| Clone Number | S-R650 |
| Antibody Type | Rat mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu, Ms |
| Positive Sample | HT29 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1:2000 | Hu, Ms |
Background
CD324, also known as E-cadherin or cadherin-1, is a calcium-dependent cell adhesion protein that plays a crucial role in maintaining epithelial tissue integrity and regulating cell proliferation, differentiation, and survival. It is a member of the cadherin superfamily and is widely expressed on the cell surface of most epithelial tissues. The extracellular domain of CD324 facilitates homophilic binding between adjacent cells, while its cytoplasmic domain interacts with the actin cytoskeleton through catenins. This protein is essential for epithelial cell cohesion and acts as an invasion suppressor, with its loss often indicating high tumor aggressiveness in cancer.
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