Flow cytometric analysis of 4% PFA fixed 0.1% Tween-20 permeabilized Mouse splenocytes, treated 6h with 80nM PMA, 1.34μM Ionomycin and 10.6μM Brefeldin A and 2μM Monensin (Right Panel) or untreated (Left Panel), labeling IL-2 at 1/200 dilution (1 μg). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with PE Rat Anti-Mouse CD3 Antibody (S0B1697) separately.
Rat Anti-Mouse IL-2 Antibody (S-R536)
Rat Anti-Mouse IL-2 Antibody (S-R536)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | IL-2 |
| Synonyms | Interleukin-2; T-cell growth factor (TCGF); Il-2; Il2 |
| Location | Secreted |
| Accession | P04351 |
| Clone Number | S-R536 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | ICFCM |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 1:200 | Ms |
Background
Interleukin-2 (IL-2) is a cytokine that plays a crucial role in the immune system. It is primarily produced by activated T cells and has multiple functions. IL-2 promotes the proliferation and differentiation of T cells, enhancing their ability to fight infections and tumors. It also stimulates the growth and activity of natural killer (NK) cells, which are important for immune defense. Additionally, IL-2 can regulate the activity of regulatory T cells (Tregs), helping to maintain immune tolerance and prevent autoimmune diseases. In clinical settings, IL-2 has been used to treat certain cancers by boosting the immune response, although its use is limited due to potential side effects. Its discovery and research have significantly advanced our understanding of the immune system and provided new strategies for immunotherapy.
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