WB result of Rat anti-Mouse
CD90.2
Antibody
Primary antibody: Rat anti-Mouse
CD90.2
Antibody at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Lane 2: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 18 kDa
Observed MW: 22 kDa
Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (S-R546)
Rat anti-Mouse CD90.2 (Thy-1.2) Antibody (S-R546)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Synonyms | THY1, T25, Thy-1, Thy-1.2 |
| Location | Cell membrane |
| Accession | P01831 |
| Clone Number | S-R546 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | WB, FCM |
| Reactivity | Ms |
| Positive Sample | mouse brain, mouse thymus, C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| FCM | 1:200 | Ms |
Background
CD90.2 protein, also known as Thy-1.2, is a 25-37 kDa glycosylphosphatidylinositol (GPI)-anchored protein with a V-type immunoglobulin domain. It is one of the two murine isoforms of the Thy-1 protein, distinguished mainly by the 89th amino acid, where Thy-1.1 carries an arginine and Thy-1.2 carries a glutamine. CD90.2 is expressed in various cell types, including T cells and fibroblasts of certain mouse strains, and plays a role in cell-to-cell and cell-matrix interactions. It is involved in axonal growth and neuronal regeneration, T cell activation and apoptosis, adhesion and migration of leukocytes and melanoma cells, as well as proliferation and migration of fibroblasts in wound healing, inflammation, and fibrosis.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of C57BL/6 mouse splenocytes labelling Mouse CD90.2 antibody at 1/200 (1 μg) dilution (Right) compared with a Rat monoclonal IgG isotype control (Left). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Brilliant Violet 421™ separately. Gated on total viable cells.
