WB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HL-60 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Rab32 Recombinant Rabbit mAb (S-1936-49)
Rab32 Recombinant Rabbit mAb (S-1936-49)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Rab32 |
| Synonyms | Ras-related protein Rab-32; RAB32 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | Q13637 |
| Clone Number | S-1936-49 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HL-60, HeLa, HepG2, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
Background
Rab32 is a small GTPase protein belonging to the RAS oncogene family, involved in various cellular processes including mitochondrial dynamics, autophagy, and vesicle trafficking. It anchors the type II regulatory subunit of protein kinase A to the mitochondrial outer membrane, facilitating mitochondrial fission. Rab32 also interacts with LRRK2, a protein implicated in Parkinson's disease, and its variants have been identified as a susceptibility factor for familial Parkinson's disease. In cancer biology, Rab32 promotes glioblastoma migration and invasion by regulating ERK/Drp1-mediated mitochondrial fission. Additionally, it plays a role in the degradation of mitochondrial-proximal ER membranes through autophagy, mediated by its effector reticulon 3L.
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Western Blot
WB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
WB result of Rab32 Recombinant Rabbit mAb
Primary antibody: Rab32 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 24 kDa
