WB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A431 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Lane 5: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
Rab11 Recombinant Rabbit mAb (S-1601-68)
Rab11 Recombinant Rabbit mAb (S-1601-68)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Rab11 |
| Synonyms | Ras-related protein Rab-11A; Rab-11; YL8; RAB11A |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P62491 |
| Clone Number | S-1601-68 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A431, HeLa, Jurkat, MCF7, A549, Neuro-2a, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
Rab11 is a member of the Rab family of small GTPases that plays a pivotal role in membrane trafficking, particularly in the recycling of membrane components from endosomes back to the plasma membrane. It is predominantly found in recycling endosomes and is crucial for the transport of vesicles from these endosomes to the cell surface, facilitating the reuse of receptors and membrane proteins. Its function has been well-defined in the recycling pathway, but its role in early endosomes is less understood. Recent studies have also implicated Rab11 in the balance of the endosome-lysosome pathway, suggesting its broader impact on cellular homeostasis. Overall, Rab11 is a key regulator in the maintenance of cellular membrane dynamics and the control of intracellular trafficking routes.
Picture
Picture
Western Blot
WB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
WB result of Rab11 Recombinant Rabbit mAb
Primary antibody: Rab11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 24 kDa
Observed MW: 24 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-Rab11 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in A549 cells. Anti-Rab11 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
