WB result of PSME4 Recombinant Rabbit mAb
Primary antibody: PSME4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 211 kDa
Observed MW: 220 kDa
PSME4 Recombinant Rabbit mAb (S-3132-44)
PSME4 Recombinant Rabbit mAb (S-3132-44)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PSME4 |
| Synonyms | Proteasome activator complex subunit 4; Proteasome activator PA200; Protein BLM10 homolog (Blm10; hBlm10); BLM10; KIAA0077; PSME4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q14997 |
| Clone Number | S-3132-44 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | 293T, K562, HeLa, HepG2, RAW264.7, mouse heart, mouse testis, mouse pancreas, C6, rat heart, rat testis |
| Predicted Reactivity | Bv, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms, Rt |
Background
PSME4 (Proteasome Activator Subunit 4), also known as PA200 or Blm10 in yeast, is a nuclear proteasome activator that uniquely stimulates the peptidase activity of the 20S core particle through a gate-opening mechanism independent of ATP and ubiquitin conjugation. Structurally, PSME4 contains an HbYX (hydrophobic-tyrosine-X) motif at its C-terminus that docks into the inter-subunit pockets of the 20S α-ring, inducing conformational changes that facilitate substrate entry into the catalytic chamber. Unlike the ATP-dependent 19S regulatory particle or the PA28/11S REG family, PSME4 forms a dome-shaped homodimer that caps the 20S proteasome and preferentially promotes the degradation of unstructured peptides and certain natively disordered proteins, including acetylated histones and unstructured regions of prion-like proteins. PSME4 plays critical roles in spermatogenesis, where it is highly expressed in testis and essential for histone-to-protamine exchange during sperm chromatin remodeling, as well as in DNA damage response and cellular stress adaptation. Dysregulation of PSME4 has been implicated in various pathological conditions, including male infertility, neurodegenerative disorders characterized by protein aggregation, and cancer, where its expression levels correlate with tumor progression and therapeutic resistance in certain malignancies.
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Western Blot
WB result of PSME4 Recombinant Rabbit mAb
Primary antibody: PSME4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Lane 3: mouse testis lysate 20 µg
Lane 4: mouse pancreas lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 211 kDa
Observed MW: 220 kDa
WB result of PSME4 Recombinant Rabbit mAb
Primary antibody: PSME4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat heart lysate 20 µg
Lane 3: rat testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 211 kDa
Observed MW: 220 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse testis. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-PSME4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in K562 cells. Anti-PSME4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in RAW 264.7 cells. Anti-PSME4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in C6 cells. Anti-PSME4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
