WB result of Prosurfactant Protein C Recombinant Rabbit mAb
Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Lane 3: mouse lung lysate 20 µg
Negative control: A549 whole cell lysate; mouse liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
Observed MW: 22 kDa
Prosurfactant Protein C Recombinant Rabbit mAb (S-1773-6)
Prosurfactant Protein C Recombinant Rabbit mAb (S-1773-6)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Prosurfactant Protein C |
| Synonyms | Surfactant protein C; SP-C; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SP5; SFTPC; SFTP2 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P11686 |
| Clone Number | S-1773-6 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | mouse lung, rat lung |
| Predicted Reactivity | Mq |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Prosurfactant Protein C (proSP-C) is a precursor protein that plays a crucial role in the formation of pulmonary surfactant, which is essential for reducing surface tension at the alveolar air-liquid interface and maintaining lung stability during ventilation. ProSP-C is synthesized as a 21 kDa integral membrane precursor protein in alveolar type II cells and is subsequently proteolytically processed to a 3.7 kDa secretory protein, known as Surfactant Protein C (SP-C). SP-C enhances the adsorption and spreading of phospholipids at the air-liquid interface, thereby promoting the surface tension-lowering properties of surfactant.
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Western Blot
WB result of Prosurfactant Protein C Recombinant Rabbit mAb
Primary antibody: Prosurfactant Protein C Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat lung lysate 20 µg
Negative control: rat liver lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 21 kDa
Observed MW: 21 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-Prosurfactant Protein C antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
