WB result of Progranulin/PGRN Recombinant Rabbit mAb
Primary antibody: Progranulin/PGRN Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: J774A.1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 63 kDa
Observed MW: 63 kDa
Progranulin/PGRN Recombinant Rabbit mAb (S-1090-110)
Progranulin/PGRN Recombinant Rabbit mAb (S-1090-110)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Progranulin/PGRN |
| Synonyms | Acrogranin; Epithelin/granulin precursor; Glycoprotein of 88 Kda (GP88; Glycoprotein 88); PC cell-derived growth factor (PCDGF); Proepithelin (PEPI); Grn |
| Immunogen | Recombinant Protein |
| Location | Secreted, Lysosome |
| Accession | P28798 |
| Clone Number | S-1090-110 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, IP |
| Reactivity | Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:500 | |
| ICC | 1:500 |
Background
Progranulin, also known as granulin-epithelin precursor (GEP), PC cell-derived growth factor (PCDGF), acrogranin, and proepithelin, is a secreted glycoprotein with a wide range of biological functions. In the context of neurodegenerative diseases, progranulin has emerged as a key factor. It is highly expressed in the brain and is implicated in the pathogenesis of frontotemporal lobar degeneration (FTLD), a type of dementia characterized by the accumulation of abnormal proteins in the brain's frontotemporal lobes. Mutations in the progranulin gene can lead to a decrease in its levels, which is associated with an increased risk of developing FTLD. Progranulin also plays a role in metabolic diseases. It has been identified as an adipokine that is involved in diet-induced obesity and insulin resistance, revealing its metabolic function. Moreover, progranulin has been linked to inflammation and immune responses. It can modulate inflammation by binding to tumor necrosis factor receptors (TNFRs) and potentially influencing TNF-dependent signaling.
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Picture
Western Blot
IP
Progranulin/PGRN Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Progranulin/PGRN in 0.4 mg J774A.1 whole cell lysate.
Western blot was performed on the immunoprecipitate using Progranulin/PGRN Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: J774A.1 whole cell lysate 20 µg (Input)
Lane 2: Progranulin/PGRN Rabbit mAb IP in J774A.1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in J774A.1 whole cell lysate
Predicted MW: 63 kDa
Observed MW: 63 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti- Progranulin/PGRN antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti- Progranulin/PGRN antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in J774A.1 cells. Anti-Progranulin/PGRN antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
