Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, left) / T-47D (Human ductal breast epithelial tumor epithelial cell, right) cells labelling Progesterone Receptor (Alexa Fluor 488 Conjugate) antibody at 1/200 (1 μg) dilution/ (red) compared with a Rabbit monoclonal IgG isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Negative control: MDA-MB-231
Progesterone Receptor Recombinant Rabbit mAb (Alex Fluor®488 Conjugate) (S-R123)
Progesterone Receptor Recombinant Rabbit mAb (Alex Fluor®488 Conjugate) (S-R123)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Progesterone Receptor |
| Synonyms | PR, NR3C3 |
| Location | Cytoplasm, Nucleus |
| Accession | P06401 |
| Clone Number | S-R123 |
| Antibody Type | Recombinant mAb |
| Application | ICC, ICFCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:500 | null |
| ICFCM | 1:200 | null |
Background
Progesterone receptor (PR) is a master regulator in female reproductive tissues that controls developmental processes and proliferation and differentiation during the reproductive cycle and pregnancy. PR also plays a role in progression of endocrine-dependent breast cancer. As a member of the nuclear receptor family of ligand-dependent transcription factors, the main action of PR is to regulate networks of target gene expression in response to binding its cognate steroid hormone, progesterone [PMID: 27380738].
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Immunocytochemistry
ICC shows positive staining in T-47D cells. Anti- Progesterone Receptor (Alex Fluor® 488 Conjugate) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
Negative control: ICC shows negative staining in MDA-MB-231 cells. Anti-Progesterone Receptor (Alex Fluor® 488 Conjugate) antibody was used at 1/500 dilution and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
