WB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HCT 116 whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: NK-92 whole cell lysate 20 µg
Lane 5: HL-60 whole cell lysate 20 µg
Negative control: HCT 116, A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 11, 12, 38, 50 kDa
pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb (S-2307-17)
pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb (S-2307-17)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | pro Caspase-1 + p10 + p12 |
| Synonyms | Caspase-1; CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE; IL-1 beta-converting enzyme); p45; IL1BC; IL1BCE; CASP1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Cell membrane |
| Accession | P29466 |
| Clone Number | S-2307-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | THP-1, NK-92, HL-60, EL4, mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
Pro Caspase-1 is a 45 kDa inactive zymogen that, upon assembly of the canonical inflammasome, undergoes autoproteolysis between the p20 and p10 subunits, releasing an N-terminal CARD domain, the p20 subunit, and the p10 subunit; the resulting heterotetramer of two p20 and two p10 chains (often described as p20-p10 plus the additional p12 peptide that can remain non-covalently associated) forms the active caspase-1 protease, which subsequently cleaves pro-IL-1β and pro-IL-18 to drive inflammatory responses.
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Western Blot
WB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 12, 14, 38, 50 kDa
WB result of pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb
Primary antibody: pro Caspase-1 + p10 + p12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45 kDa
Observed MW: 12, 14, 38, 40, 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse lung. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded mouse cerebral cortex. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-pro Caspase-1 + p10 + p12 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
