WB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDa
PPIF/Cyclophilin F Recombinant Rabbit mAb (S-2043-61)
PPIF/Cyclophilin F Recombinant Rabbit mAb (S-2043-61)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PPIF/Cyclophilin F |
| Synonyms | Peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; Cyclophilin D (CyP-D; CypD); Mitochondrial cyclophilin (CyP-M); Rotamase F; CYP3 |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P30405 |
| Clone Number | S-2043-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, HEK-293, HepG2, MCF7, mouse heart, rat heart |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu |
| ICC | 1:500 | Hu |
Background
PPIF/Cyclophilin F protein is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family, which catalyzes the isomerization of proline imide peptide bonds in oligopeptides, thereby accelerating protein folding. It is a critical component of the mitochondrial permeability transition pore (mPTP) in the inner mitochondrial membrane, and its activation is associated with the induction of apoptotic and necrotic cell death. Cyclophilin F also interacts with mitochondrial p53/TP53 to activate necrosis induced by oxidative stress and regulates mitochondrial membrane F(1)F(0) ATP synthase activity. Additionally, it has anti-apoptotic activity independent of mPTP, cooperating with BCL2 to inhibit cytochrome c-dependent apoptosis.
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Western Blot
WB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDa
WB result of PPIF/Cyclophilin F Recombinant Rabbit mAb
Primary antibody: PPIF/Cyclophilin F Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 22 kDa
Observed MW: 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-PPIF/Cyclophilin F antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-PPIF/Cyclophilin F antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
