WB result of Podoplanin Recombinant Rat mAb
Primary antibody: Podoplanin Recombinant Rat mAb at 1/1000 dilution
Lane 1: mouse lung lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 17 kDa
Observed MW: 36 kDa
Podoplanin Recombinant Rat mAb (S-3419)
Podoplanin Recombinant Rat mAb (S-3419)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Podoplanin |
| Synonyms | Aggrus; Glycoprotein 36 (Gp36); PA2.26 antigen; T1-alpha (T1A); GP36; PDPN |
| Location | Cell membrane, Cytoplasm |
| Accession | Q86YL7 |
| Clone Number | S-3419 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | WB, IHC-P, IF |
| Reactivity | Ms |
| Positive Sample | Mouse lung |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms |
| IHC-P | 1:250 | Ms |
| IF | 1:100 | Ms |
Background
Podoplanin is a small mucin-type O-glycosylated type-I transmembrane protein of 162 amino acids, highly expressed on lymphatic endothelium, alveolar type I cells, tumor cells and brain neurons, where its extracellular domain—bearing the platelet-aggregation-stimulating PLAG motif and sialylated O-glycans—engages CLEC-2 on platelets to trigger platelet activation, binds CD44 and galectin-8 to promote directional cell migration and invadopodia maturation, associates with ERM proteins (ezrin-radixin-moesin) to reorganize the actin cytoskeleton and drive epithelial-mesenchymal transition, and is indispensable for developmental separation of blood and lymphatic vessels, normal alveolus formation, lymphatic vessel integrity and hippocampus-dependent synaptic plasticity and memory, while its overexpression in cancers fosters invasion, metastasis and thrombotic risk, making it a key diagnostic and therapeutic target.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse lung. Anti-Podoplanin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse lung. Anti- Podoplanin antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rat IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
