IHC shows positive staining in paraffin-embedded human placenta. Anti-Placental lactogen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Placental lactogen Recombinant Rabbit mAb (S-454-142)
Placental lactogen Recombinant Rabbit mAb (S-454-142)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Placental lactogen |
| Synonyms | Chorionic somatomammotropin hormone 1, Choriomammotropin, Lactogen, Placental lactogen (PL), CSH1 |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P0DML2 |
| Clone Number | S-454-142 |
| Antibody Type | Rabbit mAb |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg.ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:2000 | |
| IF | 1:250 |
Background
Placental lactogen, also called chorionic somatomammotropin, is a polypeptide placental hormone, part of the somatotropin family. Its structure and function is similar to that of growth hormone. It modifies the metabolic state of the mother during pregnancy to facilitate the energy supply of the fetus.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Placental lactogen antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded human placenta. Anti-Placental lactogen antibody was used at 1/250 dilution (Green). Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
