WB result of PKR Recombinant Rabbit mAb
Primary antibody: PKR Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: HepG2 whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 38, 70 kDa
PKR Recombinant Rabbit mAb (S-731-118)
PKR Recombinant Rabbit mAb (S-731-118)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PKR |
| Synonyms | Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2); Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated (PKR; Protein kinase R); Tyrosine-protein kinase EIF2AK2; p68 kinase; PRKR; EIF2AK2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P19525 |
| Clone Number | S-731-118 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Positive Sample | MCF7, HEK-293, K562, HeLa, HepG2, Jurkat |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
PKR, also known as Protein Kinase R or double-stranded RNA-dependent protein kinase, is a serine/threonine catalytic enzyme that plays a crucial role in innate immune responses against viral infections. It is an interferon-induced kinase that can be activated by double-stranded RNA (dsRNA), a common byproduct of viral replication. Once activated, PKR phosphorylates the eukaryotic initiation factor 2α (eIF2α), which inhibits protein synthesis and viral replication. Additionally, PKR can activate the NF-κB signaling pathway, enhancing the expression of antiviral genes. Beyond its antiviral functions, PKR is involved in various cellular processes, including apoptosis, cell growth, and differentiation. Its dysregulation has been implicated in several diseases, including cancer, making it a potential therapeutic target.
Picture
Picture
Western Blot
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti- PKR antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
