WB result of PI3 Kinase p85 beta Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p85 beta Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 85 kDa
PI3 Kinase p85 beta Recombinant Rabbit mAb (S-1058-266)
PI3 Kinase p85 beta Recombinant Rabbit mAb (S-1058-266)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | PI3 Kinase p85 beta |
| Synonyms | Phosphatidylinositol 3-kinase regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3K regulatory subunit beta; PtdIns-3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta (PI3-kinase subunit p85-beta; PtdIns-3-kinase regulatory subunit p85-beta); PIK3R2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | O00459 |
| Clone Number | S-1058-266 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, Jurkat, HEK-293 |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
PI3 Kinase p85 beta is a regulatory subunit of the phosphoinositide 3-kinase (PI3K) complex, which is crucial for generating 3-phosphorylated phosphoinositides that initiate a wide range of signaling pathways. Unlike its counterpart p85 alpha, p85 beta has both overlapping and unique functions. While p85 beta alone does not significantly impact B cell development, it can partially compensate for the loss of p85 alpha in B cell development and peripheral survival. Additionally, p85 beta has been shown to have distinct effects on apoptosis and may play a role in oncogenic transformation in certain cell types. Its presence is essential for maintaining proper signaling balance, as its deletion can lead to increased BCR-mediated proliferation and altered ERK phosphorylation.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-PI3 Kinase p85 beta antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p85 beta antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
