WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: K562 whole cell lysate 40 µg
Lane 2: MCF7 whole cell lysate 40 µg
Lane 3: HeLa whole cell lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 100~130 kDa
PI3 Kinase p110β Recombinant Rabbit mAb (S-1193-109)
PI3 Kinase p110β Recombinant Rabbit mAb (S-1193-109)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform, PI3-kinase subunit beta, PI3K-beta, PI3Kbeta, PtdIns-3-kinase subunit beta, Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, PtdIns-3-kinase subunit p110-beta, p110beta, Serine/threonine protein kinase PIK3CB, PIK3CB, PIK3C1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P42338 |
| Clone Number | S-1193-109 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| ICC | 1:200 |
Background
PI3 Kinase p110 β, also known as PIK3CB, is a catalytic subunit of the phosphoinositide 3-kinase (PI3K) family, which plays a critical role in cellular processes such as growth, metabolism, and survival. PI3Ks are enzymes that generate phosphoinositol lipids as second messengers, initiating multiple signal transduction cascades. The p110 β isoform has been shown to have distinct functions in different cellular contexts and contributes to cell growth and development, DNA replication, insulin sensitivity, tumorigenesis, and G-protein-coupled receptor-activated signaling.
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Western Blot
WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 40 µg
Lane 2: mouse brain lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110~150 kDa
WB result of PI3 Kinase p110 β Recombinant Rabbit mAb
Primary antibody: PI3 Kinase p110 β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 40 µg
Lane 2: rat brain lysate 40 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 110~150 kDa
Immunocytochemistry
ICC shows positive staining in C2C12 cells. Anti- PI3 Kinase p110 β antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in HeLa cells. Anti- PI3 Kinase p110 β antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
