WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HEK-293 whole cell lysate 20 µg
Lane 2: HEK-293 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 52 kDa
This blot was developed with high sensitivity substrate
Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb (S-3537)
Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb (S-3537)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-SAPK/JNK (Thr183/Tyr185) |
| Synonyms | Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c (SAPK1c); Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; JNK1; PRKM8; SAPK1; SAPK1C; MAPK8 |
| Location | Nucleus |
| Accession | P45983 |
| Clone Number | S-3537 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Dm, Sc |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
Background
Phospho-SAPK/JNK (Thr183/Tyr185) refers to the activated form of Stress-Activated Protein Kinase/c-Jun N-terminal Kinase (SAPK/JNK) that is simultaneously phosphorylated at its threonine 183 (Thr183) and tyrosine 185 (Tyr185) residues. As a key member of the Mitogen-Activated Protein Kinase (MAPK) family, it is primarily activated by environmental stress signals such as ultraviolet radiation, gamma rays, inflammatory cytokines, and oxidative stress. Its activation depends on the dual phosphorylation of these two critical residues, Thr183 and Tyr185, by upstream kinases MKK4 or MKK7. This modification acts like a "molecular switch," altering JNK's conformation from an inactive to an active state. Once activated, Phospho-SAPK/JNK (Thr183/Tyr185) dimerizes and translocates into the nucleus. Inside the nucleus, it phosphorylates transcription factors like c-Jun and ATF-2, thereby regulating the expression of a suite of genes involved in cellular stress response, apoptosis, inflammation, and even proliferation and differentiation. Consequently, this specific phosphorylated form of the protein is not only a direct marker for JNK pathway activation, but its dysregulated activity is also closely linked to various pathological processes, including neurodegenerative diseases, inflammatory conditions, and cancer, making it a significant target in life science research and the exploration of disease mechanisms.
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Western Blot
WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 52 kDa
This blot was developed with high sensitivity substrate
WB result of Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-SAPK/JNK (Thr183/Tyr185) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with UV (40mJ/cm2, 30 minutes recovery) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 40, 52 kDa
This blot was developed with high sensitivity substrate
