WB result of Phospho-PKR (Thr446) Recombinant Rabbit mAb
Primary antibody: Phospho-PKR (Thr446) Recombinant Rabbit mAb at 1/500 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 100 ng/ml Calyculin A for 30 minutes and 20 ng/ml TNF-α for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 70 kDa
Phospho-PKR (Thr446) Recombinant Rabbit mAb (S-771-17)
Phospho-PKR (Thr446) Recombinant Rabbit mAb (S-771-17)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PKR (Thr446) |
| Synonyms | Interferon-induced, double-stranded RNA-activated protein kinase; Eukaryotic translation initiation factor 2-alpha kinase 2 (eIF-2A protein kinase 2); Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; Protein kinase RNA-activated (PKR; Protein kinase R); Tyrosine-protein kinase EIF2AK2; p68 kinase; PKR; PRKR; EIF2AK2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P19525 |
| Clone Number | S-771-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:500 | Hu |
Background
Protein Kinase R (PKR), also known as double-stranded RNA-dependent protein kinase, is a key enzyme involved in the innate immune response to viral infections. It is an interferon-induced, serine/threonine protein kinase that becomes activated upon binding to double-stranded RNA (dsRNA), which is often produced during viral replication. Once activated, PKR phosphorylates the eukaryotic initiation factor 2α (eIF-2α), inhibiting protein synthesis and thus limiting viral replication. Additionally, PKR plays roles in regulating apoptosis, cell growth, and differentiation, and has been implicated in cancer and metabolic diseases. Its versatile functions make it a significant target for therapeutic research.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-PKR (Thr446) Recombinant Rabbit mAb
Lane 1: PKR (Thr446) phospho peptide
Lane 2: PKR (Thr446) unmodified peptide
Primary antibody: Phospho-PKR (Thr446) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
