WB result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 0.05 µM PMA for 12 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 78 kDa
Observed MW: 78 kDa
Phospho-PKCδ (Thr505) Recombinant Rabbit mAb (S-1668-89)
Phospho-PKCδ (Thr505) Recombinant Rabbit mAb (S-1668-89)
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$130.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PKCδ (Thr505) |
| Synonyms | Protein kinase C delta type; Tyrosine-protein kinase PRKCD; nPKC-delta; PKCD; PRKCD |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion, Cell membrane |
| Accession | Q05655 |
| Clone Number | S-1668-89 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | THP-1 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
Background
Phospho-PKCδ (Thr505) refers to the phosphorylation of protein kinase C delta (PKCδ) at threonine residue 505, a modification that is crucial for its activation and function. PKCδ is a member of the protein kinase C (PKC) family, which are serine/threonine kinases involved in various cellular signaling pathways, including regulation of cell growth, apoptosis, and differentiation. The phosphorylation at Thr505 is particularly important for the kinase activity of PKCδ and its role in the G1 to S phase transition during cell cycle progression. This phosphorylation site is specific to PKCδ, and antibodies targeting Phospho-PKCδ (Thr505) do not cross-react with other PKC isoforms, making it a valuable marker for studying PKCδ's unique functions.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Phospho-PKCδ (Thr505) Recombinant Rabbit mAb
Lane 1: PKC
δ
(Thr505) phospho peptide
Lane 2: PKC
δ
(Thr505) unmodified peptide
Primary antibody: Phospho-PKCδ (Thr505) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
