WB result of Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
Phospho-PKA Substrate (RRXST) Recombinant Rabbit mAb (S-2623-148)
Phospho-PKA Substrate (RRXST) Recombinant Rabbit mAb (S-2623-148)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PKA Substrate (RRXST) |
| Immunogen | Synthetic Peptide |
| Clone Number | S-2623-148 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, WB |
| Reactivity | Hu, Ms |
| Positive Sample | starve overnight and Calyculin A treated HeLa, Calyculin A treated NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms |
Background
Phospho-PKA Substrate (RRXST) is a recognition motif for substrates specifically phosphorylated by protein kinase A (PKA). Within this motif, serine (S) or threonine (T) serves as the phosphorylation site, flanked on its N-terminal side by two arginine residues (RR). The position immediately following the second arginine can be any amino acid (X). This highly conserved pattern of basic amino acids ensures that PKA can specifically recognize and catalyze the phosphorylation of serine/threonine residues in its substrates, thereby regulating various critical cellular signaling pathways, including glycogen metabolism, gene transcription, and cell proliferation. Specific phospho-antibodies targeting this motif have become essential research tools for detecting PKA activity and its downstream signaling events.
Picture
Picture
Western Blot
WB result of Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-PKA Substrate (RRXS*/T*) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: Multiple
Observed MW: Multiple
ELISA
ELISA binding assay shows Phospho-PKA Substrate (RRXST) Antibody specifically binds to phospho and non-phospho motif peptides.
