WB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDa
Phospho-PDHA1 (S293) Recombinant Rabbit mAb (S-1729-25)
Phospho-PDHA1 (S293) Recombinant Rabbit mAb (S-1729-25)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PDHA1 (S293) |
| Synonyms | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; PHE1A |
| Immunogen | Synthetic Peptide |
| Location | Mitochondrion |
| Accession | P08559 |
| Clone Number | S-1729-25 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, HEK-293, HeLa, NIH/3T3, mouse brain, PC-12, Rat brain |
| Predicted Reactivity | Bv, CyMk, Or, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
PDHA1, or pyruvate dehydrogenase E1 subunit alpha 1, is a crucial component of the pyruvate dehydrogenase complex (PDHc), which is a mitochondrial multienzyme complex that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, providing a primary link between glycolysis and the tricarboxylic acid (TCA) cycle. Phosphorylation of PDHA1 at Ser293 (PDHA1 phospho S293) is an important regulatory modification that affects the activity of the PDH complex, which is crucial for the regulation of glucose metabolism and fatty acid oxidation, which are sensitive targets for insulin action.
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Western Blot
WB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: phosphatase treated HeLa whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDa
WB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDa
WB result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 43 kDa
Observed MW: 43 kDa
Dot Blot
Dot blot result of phospho-PDHA1 (S293) Recombinant Rabbit mAb
Lane 1: PDHA1 (S293) phospho peptide
Lane 2: PDHA1 unmodified peptide
Primary antibody: phospho-PDHA1 (S293) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma and negative staining in cervical squamous cell carcinoma treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney and negative staining in human kidney treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach and negative staining in mouse stomach treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle and negative staining in rat cardiac muscle treated with phosphatase. Anti- Phospho-PDHA1 (S293) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
