WB result of Phospho-PDGF Receptor α (Tyr1018) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody incubation conditions: overnight at 4°C
Primary antibody: Phospho-PDGF Receptor α (Tyr1018) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starved overnight, then treated with 100 ng/ml PDGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 123 kDa
Observed MW: 190 kDa
Phospho-PDGF Receptor α (Tyr1018) Recombinant Rabbit mAb (S-3482-7)
Phospho-PDGF Receptor α (Tyr1018) Recombinant Rabbit mAb (S-3482-7)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-PDGF Receptor α (Tyr1018) |
| Synonyms | Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 2 (PDGFR-2); CD140a; PDGFR2; RHEPDGFRA; PDGFRA |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P16234 |
| Clone Number | S-3482-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Positive Sample | NIH/3T3 (starved overnight, then treated with 100 ng/ml PDGF for 5 minutes) |
| Predicted Reactivity | Hu, Rt, Ck, Xe |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
Background
Phospho-PDGF Receptor α (Tyr1018) is the activated form of the platelet-derived growth factor receptor alpha (PDGFRα) following phosphorylation at tyrosine residue 1018, representing the activation state of this receptor tyrosine kinase signaling pathway. This site is located within the kinase insert region of PDGFRα. Upon ligand binding (such as PDGF-AA, PDGF-BB, or PDGF-CC), the receptor undergoes dimerization and autophosphorylation, with phosphorylation at Tyr1018 providing a critical docking site for the signaling adaptors Grb2 and Shc, thereby activating the Ras-MAPK pathway to regulate cell proliferation, survival, and migration. Studies have shown that aberrant phosphorylation at Tyr1018 is closely associated with sustained activation of PDGFRα in conditions such as idiopathic pulmonary fibrosis, atherosclerosis, and various tumors (e.g., glioblastoma, gastrointestinal stromal tumors).
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Western Blot
