WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 20 ng/ml TNF-alpha and 50 nM Calyculin A for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
Observed MW: 50, 110 kDa
Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb (S-1127-19)
Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb (S-1127-19)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-NF-kB p105/p50 (Ser337) |
| Synonyms | DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P19838 |
| Clone Number | S-1127-19 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Ck, Dg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:5000 | Hu, Ms, Rt |
Background
Phospho-NF-kB p105/p50 (Ser337) is a phosphorylated form of the NF-kB subunits p105 and p50, which are crucial in the regulation of gene expression, particularly in response to various stimuli such as inflammation, immunity, differentiation, cell growth, and tumorigenesis. Phosphorylation at Ser337 is significant for the transcriptional activity of these subunits. It has been shown that phosphorylation at this site can affect the dimerization of p50 and its interaction with DNA. Specifically, phosphorylation by protein kinases like PKAc and Chk1 influences the ability of p50 to form homodimers and bind to DNA, which in turn modulates the transcriptional output of genes under the control of NF-kB. This phosphorylation event is also implicated in the gene-specific regulation of transcription, offering opportunities for selective targeting of NF-kB for therapeutic benefits. Thus, Phospho-NF-kB p105/p50 (Ser337) is a critical regulatory point in the NF-kB signaling pathway, impacting the transcriptional program that governs key physiological processes and disease states.
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Western Blot
WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 20 ng/ml TNF-alpha and 100 nM Calyculin A for 10 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
Observed MW: 55 kDa
WB result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 100 nM Calyculin A for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 105 kDa
Observed MW: 60, 110 kDa
Dot Blot
Dot blot result of Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb
Lane 1: NF-kB p105/p50 (Ser337) phospho peptide
Lane 2: NF-kB p105/p50 unmodified peptide
Primary antibody: Phospho-NF-kB p105/p50 (Ser337) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
