WB result of Phospho-mTOR (Ser2448) Rabbit mAb
Primary antibody: Phospho-mTOR (Ser2448) Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa starve overnight, then treated with PMA (200 nM, 4 h) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 260 kDa
Exposure time: 180 s
Phospho-mTOR (Ser2448) Recombinant Rabbit mAb (S-705-7)
Phospho-mTOR (Ser2448) Recombinant Rabbit mAb (S-705-7)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P42345 |
| Clone Number | S-705-7 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 |
Background
The mammalian target of rapamycin (mTOR), also referred to as the mechanistic target of rapamycin, and sometimes called FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that in humans is encoded by the MTOR gene. mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases. mTOR links with other proteins and serves as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, which regulate different cellular processes. In particular, as a core component of both complexes, mTOR functions as a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, autophagy, and transcription. As a core component of mTORC2, mTOR also functions as a tyrosine protein kinase that promotes the activation of insulin receptors and insulin-like growth factor 1 receptors. mTORC2 has also been implicated in the control and maintenance of the actin cytoskeleton.
Picture
Picture
Western Blot
WB result of Phospho-mTOR (Ser2448) Rabbit mAb
Primary antibody: Phospho-mTOR (Ser2448) Rabbit mAb at 1/1000 dilution
Lane 1: untreated PC-12 whole cell lysate 20 µg
Lane 2: PC-12 starve overnight, then treated with insulin (150 nM, 5min) whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 289 kDa
Observed MW: 260 kDa
Dot Blot
Dot blot result of Phospho-mTOR (Ser2448) Rabbit mAb
Lane 1: Phospho-mTOR (Ser2448) peptide
Lane 2: mTOR unmodified peptide
Primary antibody: Phospho-mTOR (Ser2448) Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Exposure time: 8 s
