WB result of Phospho-IRAK4 (Thr345/Ser346) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-IRAK4 (Thr345/Ser346) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated Karpas-299 whole cell lysate 20 µg
Lane 2: Karpas-299 treated with 50 ng/ml human Interleukin-1b for 15 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52 kDa
Observed MW: 52 kDa
Phospho-IRAK4 (Thr345/Ser346) Recombinant Rabbit mAb (S-3288)
Phospho-IRAK4 (Thr345/Ser346) Recombinant Rabbit mAb (S-3288)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-IRAK4 (Thr345/Ser346) |
| Synonyms | Interleukin-1 receptor-associated kinase 4; IRAK-4; Renal carcinoma antigen NY-REN-64; IRAK4 |
| Location | Cytoplasm |
| Accession | Q9NWZ3 |
| Clone Number | S-3288 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-IRAK4 (Thr345/Ser346) is the autophosphorylated, catalytically active form of interleukin-1 receptor-associated kinase 4 that forms within minutes of TLR/IL-1R stimulation; these two adjacent residues in the activation loop must be phosphorylated—chiefly through IRAK4 trans-autophosphorylation after MyD88-mediated dimerization—to stabilize the loop, fully unleash kinase activity toward IRAK1 and Pellino E3 ligases, and thereby propagate downstream NF-κB and MAPK inflammatory signaling.
Picture
Picture
Western Blot
