WB result of Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated 293T whole cell lysate 20 µg
Lane 2: 293T treated with 100 nM insulin for 20 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 45, 53 kDa
Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb (S-3304)
Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb (S-3304)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-GSK3a/β (Ser21/9) |
| Synonyms | Glycogen synthase kinase-3 alpha; GSK-3 alpha; Serine/threonine-protein kinase GSK3A; GSK3A; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B; GSK3B |
| Location | Cytoplasm, Nucleus, Mitochondrion, Synapse |
| Accession | P49840、P49841 |
| Clone Number | S-3304 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
Background
Phospho-GSK3α/β (Ser21/9) is the phosphorylated, inactive form of glycogen synthase kinase-3 alpha and beta isoforms, generated when AKT, MAPK and other kinases add a phosphate to Ser21 of GSK3α or the homologous Ser9 of GSK3β, thereby creating an N-terminal pseudo-substrate that folds back into the catalytic pocket to block substrate access and halt the enzyme’s constitutive suppression of glycogen synthesis, β-catenin signaling, NF-κB activity and other metabolic/transcriptional programs; detection of this phospho-epitope therefore serves as a widely used biomarker for PI3K–AKT–mTOR pathway activation and for cellular responses to insulin, growth factors, oxidative stress and Wnt stimuli.
Picture
Picture
Western Blot
WB result of Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-GSK3a/β (Ser21/9) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 starve overnight, then treated with 100 ng/ml PDGF for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 47, 51 kDa
Observed MW: 45, 53 kDa
This blot was developed with high sensitivity substrate
