WB result of Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated MDA-MB-231 whole cell lysate 20 µg
Lane 2: MDA-MB-231 starve overnight, then treated with 100 ng/ml Ephrin-A for 5 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 110 kDa
Observed MW: 120 kDa
Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb (S-3551)
Phospho-EphA3 (Tyr779) Recombinant Rabbit mAb (S-3551)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-EphA3 (Tyr779) |
| Synonyms | Ephrin type-A receptor 3; EPH-like kinase 4 (EK4; hEK4); HEK (Human embryo kinase); Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1 (Eph-like tyrosine kinase 1); ETK; ETK1; HEK; TYRO4; EPHA3 |
| Location | Secreted, Cell membrane |
| Accession | P29320 |
| Clone Number | S-3551 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-EphA3 (Tyr779) is the ligand-triggered, autophosphorylated form of the receptor tyrosine kinase EphA3 that localizes within the kinase domain; its phosphorylation is essential for full receptor activation, creates a docking site for the SH2-domain adaptor CrkII, and thereby couples EphA3 to downstream RhoA/ROCK signaling to regulate actomyosin dynamics, cell adhesion, migration and neurite retraction.
Picture
Picture
Western Blot
