WB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated HeLa whole cell lysate 20 µg
Lane 2: HeLa treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Phospho-eIF4E (Ser209) Recombinant Rabbit mAb (S-2652-195)
Phospho-eIF4E (Ser209) Recombinant Rabbit mAb (S-2652-195)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Phospho-eIF4E (Ser209) |
| Synonyms | Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; EIF4EL1; EIF4F |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P06730 |
| Clone Number | S-2652-195 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Predicted Reactivity | Bv, Xe |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:500 | Hu, Ms, Rt |
Background
Phospho-eIF4E (Ser209) refers to the activated form of the eukaryotic translation initiation factor 4E following the phosphorylation of its serine residue at position 209. This event is a critical regulatory step in controlling the initiation of protein synthesis. eIF4E itself is the key initiation factor that recognizes and binds to the 5' cap structure of mRNA, serving as the core component of the eIF4F complex responsible for recruiting the ribosome to the mRNA. When its Ser209 site is phosphorylated by the specific kinases MNK1 and MNK2, the affinity of eIF4E for the cap structure is significantly enhanced, and its binding to eIF4G is promoted. This process preferentially accelerates the translation efficiency of a specific class of mRNAs, which typically possess highly complex 5' untranslated regions and encode proteins crucial for cell proliferation, survival, migration, and angiogenesis (such as c-Myc, Cyclin D1, and VEGF). This phosphorylation event is a key downstream effect of the Ras/MAPK signaling pathway. Notably, in cancer, the level of Phospho-eIF4E (Ser209) is often abnormally elevated, leading to increased synthesis of oncogenic proteins that drive tumor initiation, progression, invasion, and resistance to chemotherapy. Consequently, it is regarded as a highly promising therapeutic target and prognostic biomarker in oncology.
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Picture
Western Blot
WB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated NIH/3T3 whole cell lysate 20 µg
Lane 2: NIH/3T3 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
WB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated C6 whole cell lysate 20 µg
Lane 2: C6 treated with 25 μg/ml Anisomycin for 30 minutes whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
WB result of Phospho-eIF4E (Ser209) Recombinant Rabbit mAb
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Primary antibody: Phospho-eIF4E (Ser209) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 25 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human prostate. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti- Phospho-eIF4E (Ser209) antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
